loading...| Preferred Name |
Translocation of BoNT Light chain |
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| Definitions |
Translocation of the BoNT Light chain (LC) from an endocytic vesicle into the cytosol is essential for neurotoxicity. It has been proposed that acidic pH within the endosome triggers a conformational change in the Heavy chain (HC) N-terminal domain enabling its insertion into the endosomal lipid bilayer membrane to form a channel large enough to accommodate the unfolded L chain. The L chain moiety of the BoNT protein then traverses this channel towards the cytosol, the disulfide bond holding the H and L chains together is broken, and the L chain refolds and is released into the cytosol. Koriazova and Montal (2003) proposed that the "LC refolds at the interface and dissociates from the HC in the reducing cytosolic milieu where it cleaves its substrate SNAP-25".The molecular details of this process are not yet well understood.<br> Authored: Krupa, S, Gopinathrao, G, 2006-06-15 10:36:11 Edited: Gopinathrao, G, 2006-06-15 22:12:29 Reviewed: Ichtchenko, K, 2007-08-03 18:17:25 |
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http://purl.obolibrary.org/obo/HINO_0015702 |
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| comment |
Translocation of the BoNT Light chain (LC) from an endocytic vesicle into the cytosol is essential for neurotoxicity. It has been proposed that acidic pH within the endosome triggers a conformational change in the Heavy chain (HC) N-terminal domain enabling its insertion into the endosomal lipid bilayer membrane to form a channel large enough to accommodate the unfolded L chain. The L chain moiety of the BoNT protein then traverses this channel towards the cytosol, the disulfide bond holding the H and L chains together is broken, and the L chain refolds and is released into the cytosol. Koriazova and Montal (2003) proposed that the "LC refolds at the interface and dissociates from the HC in the reducing cytosolic milieu where it cleaves its substrate SNAP-25".The molecular details of this process are not yet well understood. Authored: Krupa, S, Gopinathrao, G, 2006-06-15 10:36:11 Edited: Gopinathrao, G, 2006-06-15 22:12:29 Reviewed: Ichtchenko, K, 2007-08-03 18:17:25 |
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| definition source |
Pubmed12417130 Pubmed8206166 Pubmed12459720 Reactome, http://www.reactome.org Pubmed13678859 Pubmed7527117 |
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| label |
Translocation of BoNT Light chain |
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| located_in | ||
| prefixIRI |
HINO:0015702 |
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| prefLabel |
Translocation of BoNT Light chain |
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| seeAlso |
Reactome Database ID Release 43181363 ReactomeREACT_11085 |
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| has_part |
http://purl.obolibrary.org/obo/HINO_0026744 |