loading...| Preferred Name | Polymerase chain reaction | |
| Synonyms |
random amplified polymorphic dna technique reaction, polymerase chain anchored pcr pcr analysis pcr, nested pcr - polymerase chain reaction anchored polymerase chain reaction polymerase chain reactions reactions, polymerase chain pcr, anchored pcr dna fingerprinting nested polymerase chain reaction nested pcr pcr assay |
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| Definitions |
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. |
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| ID |
https://pat.nichd.nih.gov/patinvestigativetechniques/IT.14 |
|
| Definition |
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. |
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| Definition Source |
MSH |
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| External Source | ||
| Label |
Polymerase chain reaction |
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| notation |
IT.14 |
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| PAT ID |
IT.14 |
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| prefLabel |
Polymerase chain reaction |
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| Sub Class Of | ||
| Synonyms |
random amplified polymorphic dna technique reaction, polymerase chain anchored pcr pcr analysis pcr, nested pcr - polymerase chain reaction anchored polymerase chain reaction polymerase chain reactions reactions, polymerase chain pcr, anchored pcr dna fingerprinting nested polymerase chain reaction nested pcr pcr assay |