Molecular Interactions

Last uploaded: August 6, 2024
Preferred Name

enzyme linked immunosorbent assay
Synonyms

ELISA

elisa

Definitions

Following non-covalent binding of a purified primary ligand to a solid phase, a blocking reagent is added to prevent any non-specific binding. A specific antigen is then allowed to bind to the primary ligand. Unbound antigen is removed by washing and a secondary antibody conjugated to an enzyme (e.g. horseradish peroxidase) is added. Following a washing step to remove unbound secondary ligand, the extent to which a chromogenic substrate (e.g. 3,3', 5,5' tetramethyl benzidine chromogen [TMB]) is converted to a soluble coloured product by the conjugated enzyme in a given time is determined by spectrophotometry using a standard microplate absorbance reader. A similar type of approach can be utilized to detect enzymatic activities. The substrate, attached to a solid phase is incubated in the presence of the enzyme and the enzymatic modification is monitored by an antibody that is specific for the modified substrate (for instance a phosphorylated protein).

ID

http://purl.obolibrary.org/obo/MI_0411

definition

Following non-covalent binding of a purified primary ligand to a solid phase, a blocking reagent is added to prevent any non-specific binding. A specific antigen is then allowed to bind to the primary ligand. Unbound antigen is removed by washing and a secondary antibody conjugated to an enzyme (e.g. horseradish peroxidase) is added. Following a washing step to remove unbound secondary ligand, the extent to which a chromogenic substrate (e.g. 3,3', 5,5' tetramethyl benzidine chromogen [TMB]) is converted to a soluble coloured product by the conjugated enzyme in a given time is determined by spectrophotometry using a standard microplate absorbance reader. A similar type of approach can be utilized to detect enzymatic activities. The substrate, attached to a solid phase is incubated in the presence of the enzyme and the enzymatic modification is monitored by an antibody that is specific for the modified substrate (for instance a phosphorylated protein).

has_exact_synonym

ELISA

elisa

has_obo_namespace

PSI-MI

id

MI:0411

in_subset

http://purl.obolibrary.org/obo/mi#PSI-MI_slim

label

enzyme linked immunosorbent assay

notation

MI:0411

prefLabel

enzyme linked immunosorbent assay

treeView

http://purl.obolibrary.org/obo/MI_0892

http://purl.obolibrary.org/obo/MI_0421

subClassOf

http://purl.obolibrary.org/obo/MI_0892

http://purl.obolibrary.org/obo/MI_0421

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Mapping To Ontology Source
http://purl.bioontology.org/ontology/RCD/X77a4 RCD LOOM
http://purl.bioontology.org/ontology/CSP/1554-7840 CRISP LOOM
http://phenomebrowser.net/ontologies/mesh/mesh.owl#D004797 RH-MESH LOOM
http://purl.obolibrary.org/obo/MMO_0000075 MMO LOOM
https://pat.nichd.nih.gov/patinvestigativetechniques/IT.08.01 PATIT LOOM
http://purl.obolibrary.org/obo/OBI_0000661 ENM LOOM
http://purl.obolibrary.org/obo/OBI_0000661 PDRO LOOM
http://purl.obolibrary.org/obo/OBI_0000661 EUPATH LOOM
http://purl.obolibrary.org/obo/OBI_0000661 OBI LOOM
http://purl.obolibrary.org/obo/OBI_0000661 ONE LOOM
http://purl.obolibrary.org/obo/OBI_0000661 OPMI LOOM
http://purl.obolibrary.org/obo/OBI_0000661 EGO LOOM
http://purl.obolibrary.org/obo/OBI_0000661 BMONT LOOM
http://purl.obolibrary.org/obo/OBI_0000661 COVID-19 LOOM
http://purl.obolibrary.org/obo/OBI_0000661 OBI_IEE LOOM
http://purl.obolibrary.org/obo/OBI_0000661 KTAO LOOM
http://phenomebrowser.net/ontologies/mesh/mesh.owl#E05.601.495.380.360 RH-MESH LOOM
http://phenomebrowser.net/ontologies/mesh/mesh.owl#E05.601.495.350.170 RH-MESH LOOM
http://purl.obolibrary.org/obo/MESH_D004797 BERO LOOM
http://purl.bioontology.org/ontology/MEDDRA/10059694 MEDDRA LOOM
http://purl.bioontology.org/ontology/MESH/D004797 MESH LOOM
http://purl.bioontology.org/ontology/PDQ/CDR0000635523 PDQ LOOM
http://purl.obolibrary.org/obo/OMIT_0006038 OMIT LOOM
http://purl.org/m4m/1347 GFF-M4M LOOM
http://phenomebrowser.net/ontologies/mesh/mesh.owl#E05.478.583.400.170 RH-MESH LOOM
http://id.nlm.nih.gov/mesh/D004797 MDM LOOM
http://purl.obolibrary.org/obo/IDOMAL_0000222 IDOMAL LOOM
http://bmi.utah.edu/ontologies/hfontology/C0014441 HFO LOOM
http://phenomebrowser.net/ontologies/mesh/mesh.owl#E05.478.566.380.360 RH-MESH LOOM
http://phenomebrowser.net/ontologies/mesh/mesh.owl#E05.478.566.350.170 RH-MESH LOOM
http://purl.bioontology.org/ontology/SNOMEDCT/76978006 SNOMEDCT LOOM