loading...| Preferred Name | Polymerase Chain Reaction | |
| Synonyms |
Inverse Polymerase Chain Reaction PCR, Anchored Inverse PCR PCR Nested PCR Anchored PCR PCR, Nested Nested Polymerase Chain Reaction Reactions, Polymerase Chain Polymerase Chain Reactions Anchored Polymerase Chain Reaction Reaction, Polymerase Chain PCR, Inverse |
|
| Definitions |
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. |
|
| ID |
http://purl.bioontology.org/ontology/MESH/D016133 |
|
| altLabel |
Inverse Polymerase Chain Reaction PCR, Anchored Inverse PCR PCR Nested PCR Anchored PCR PCR, Nested Nested Polymerase Chain Reaction Reactions, Polymerase Chain Polymerase Chain Reactions Anchored Polymerase Chain Reaction Reaction, Polymerase Chain PCR, Inverse |
|
| AQL |
CL EC ES HI IS MT SN ST TD VE |
|
| cui |
C0242574 C0079639 C0032520 C0078945 |
|
| DC |
1 |
|
| definition |
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. |
|
| DX |
19910101 |
|
| FX |
D016324 D022521 |
|
| HN |
91(90); was see under GENE AMPLIFICATION 1990 |
|
| Inverse of AQ |
http://purl.bioontology.org/ontology/MESH/Q000266 http://purl.bioontology.org/ontology/MESH/Q000379 http://purl.bioontology.org/ontology/MESH/Q000706 http://purl.bioontology.org/ontology/MESH/Q000662 http://purl.bioontology.org/ontology/MESH/Q000941 http://purl.bioontology.org/ontology/MESH/Q000191 http://purl.bioontology.org/ontology/MESH/Q000145 http://purl.bioontology.org/ontology/MESH/Q000592 |
|
| Inverse of RO | ||
| Machine permutation |
91; was see under GENE AMPLIFICATION 1990 |
|
| MDA |
19900111 |
|
| MMR |
20110624 |
|
| MN |
E05.393.620.500 |
|
| notation |
D016133 |
|
| prefLabel |
Polymerase Chain Reaction |
|
| TERMUI |
T047910 T047911 T047909 T047908 T047915 T047913 T047914 T047912 |
|
| TH |
NLM (1991) NLM (1995) NLM (1990) |
|
| tui |
T063 |
|
| subClassOf |