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The Biomarker Ontology
Last uploaded:
June 3, 2024
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| Preferred Name | enzyme-linked immunosorbent assay | |
| Synonyms |
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| Definitions |
An analyte assay where binding of an enzyme linked antibody to a material entity that is immobilized on solid support is detected utilizing a chemiluminescent reaction. Depending on the setup, the enzyme-linked antibody could be binding directly to the analyte, or it serves as a secondary antibody detecting binding of the primary antibody to the analyte. |
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| ID |
http://purl.obolibrary.org/obo/OBI_0000661 |
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| alternative term |
ELISA
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| definition |
An analyte assay where binding of an enzyme linked antibody to a material entity that is immobilized on solid support is detected utilizing a chemiluminescent reaction. Depending on the setup, the enzyme-linked antibody could be binding directly to the analyte, or it serves as a secondary antibody detecting binding of the primary antibody to the analyte.
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| definition_source |
IEDB
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| editor preferred term |
enzyme-linked immunosorbent assay
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| example of usage |
1) Detection of IL-2 (analyte) in a cell supernatant (evaluant), using plate bound anti IL-2 antibodies, and a reporter enzyme-linked reporter antibody. 2) Measurement of IgG antibody (analyte) titer in a serum sample (evaluant) using plate bound antigen and a reporter anti-IgG antibody.
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| has curation status | ||
| imported from | ||
| label |
enzyme-linked immunosorbent assay
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| note |
An analyte assay where binding of an enzyme linked antibody to a material entity that is immobilized on solid support is detected utilizing a chemiluminescent reaction. Depending on the setup, the enzyme-linked antibody could be binding directly to the analyte, or it serves as a secondary antibody detecting binding of the primary antibody to the analyte.
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| preferred label |
enzyme-linked immunosorbent assay
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| prefixIRI |
OBI:0000661
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| prefLabel |
enzyme-linked immunosorbent assay
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| term editor |
IEDB
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| textual definition |
An analyte assay where binding of an enzyme linked antibody to a material entity that is immobilized on solid support is detected utilizing a chemiluminescent reaction. Depending on the setup, the enzyme-linked antibody could be binding directly to the analyte, or it serves as a secondary antibody detecting binding of the primary antibody to the analyte.
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