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BioAssay Ontology
Last uploaded:
February 21, 2024
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| Preferred Name | immunohistochemistry | |
| Synonyms |
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| Definitions |
This technique is used to detect proteins and peptides in a tissue section by binding to specific antibodies. Subsequently, a secondary antibody that is conjugated to a fluorophore or an enzyme needed for the detection of a signal is hybridized to the primary antibody. Signal is generally detected by immunofluorescence or less commonly, by a colored reaction. This allows the detection and subcellular localization of the protein of interest by visualization under a microscope. |
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| ID |
http://www.bioassayontology.org/bao#BAO_0000415 |
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| label |
immunohistochemistry
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| prefixIRI |
bao:BAO_0000415
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| prefLabel |
immunohistochemistry
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| textual definition |
This technique is used to detect proteins and peptides in a tissue section by binding to specific antibodies. Subsequently, a secondary antibody that is conjugated to a fluorophore or an enzyme needed for the detection of a signal is hybridized to the primary antibody. Signal is generally detected by immunofluorescence or less commonly, by a colored reaction. This allows the detection and subcellular localization of the protein of interest by visualization under a microscope.
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