Link to this page
The Prescription of Drugs Ontology
Last uploaded:
September 4, 2020
Jump to:
| Preferred Name | western blot assay | |
| Synonyms |
|
|
| Definitions |
An analyte assay that detects specific peptides in an input material by separating it using gel electrophoresis, transfering the separated molecules to a membrane, and staining them with_ antibodies specific to the analyte molecules. |
|
| ID |
http://purl.obolibrary.org/obo/OBI_0000854 |
|
| definition source |
Philippe Rocca-Serra IEDB
|
|
| example of usage |
Running a cell lysate on an acrylamide gel in a western blot aparatus to separate the constituent proteins, followed by transfer of the proteins from the gel to a nitrocellulose membrane. Staining this membrane with specific antibodies to detect the presence of specific proteins of interest.
|
|
| label |
western blot assay
|
|
| prefixIRI |
OBI:0000854
|
|
| prefLabel |
western blot assay
|
|
| term editor |
Philippe Rocca-Serra IEDB
|
|
| textual definition |
An analyte assay that detects specific peptides in an input material by separating it using gel electrophoresis, transfering the separated molecules to a membrane, and staining them with_ antibodies specific to the analyte molecules.
|
|
| subClassOf |
Add comment
| Delete | Subject | Author | Type | Created |
|---|---|---|---|---|
| No notes to display |
Create mapping