Link to this page
Ontology for Nutritional Epidemiology
Last uploaded:
March 25, 2021
Jump to:
| Preferred Name | enzyme-linked immunospot assay | |
| Synonyms |
|
|
| Definitions |
A cytometry assay in which cells are cultured on a surface coated with a capture antibody binding a secretory material of interest which subsequently gets stained resulting in a spot for each cell producing the secretory material of interest. |
|
| ID |
http://purl.obolibrary.org/obo/OBI_0600031 |
|
| alternative term |
ELISPOT assay
|
|
| definition |
A cytometry assay in which cells are cultured on a surface coated with a capture antibody binding a secretory material of interest which subsequently gets stained resulting in a spot for each cell producing the secretory material of interest.
|
|
| definition source |
IEDB
|
|
| editor preferred term |
enzyme-linked immunospot assay
|
|
| example of usage |
Determination of the frequency of cells producing IFN-gamma in response to viral peptides by placing effector cells on a anti IFN-gamma coated plate, and adding antigen presenting cells pulsed with a peptide. After washing, the bound IFN-gamma is detected with a secondary antibody linked to a dye that visualizes as one spot per cell.
|
|
| has curation status | ||
| label |
enzyme-linked immunospot assay
|
|
| prefixIRI |
OBI:0600031
|
|
| prefLabel |
enzyme-linked immunospot assay
|
|
| term editor |
IEDB
|
|
| subClassOf |
Add comment
| Delete | Subject | Author | Type | Created |
|---|---|---|---|---|
| No notes to display |
Create mapping