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Ontology for Nutritional Epidemiology
Last uploaded:
March 25, 2021
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| Preferred Name | Southern blot assay | |
| Synonyms |
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| Definitions |
An analyte assay that detects the presence of a specific sequence in a DNA sample, which has been digested by restriction enzymes, resolved by gel electrophoresis, and blotted to a solid support, followed by hybridization of a probe raised against a specific sequence and detected with fluorescence or radioactivity. |
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| ID |
http://purl.obolibrary.org/obo/OBI_0000892 |
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| alternative term |
Southern blot
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| definition |
An analyte assay that detects the presence of a specific sequence in a DNA sample, which has been digested by restriction enzymes, resolved by gel electrophoresis, and blotted to a solid support, followed by hybridization of a probe raised against a specific sequence and detected with fluorescence or radioactivity.
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| definition source |
OBI
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| editor preferred term |
Southern blot assay
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| example of usage |
PMID: 9452032. Germline mutations detected in the von Hippel-Lindau disease tumor suppressor gene by Southern blot and direct genomic DNA sequencing.
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| has curation status | ||
| has part | ||
| has participant | ||
| has_specified_output | ||
| label |
Southern blot assay
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| prefixIRI |
OBI:0000892
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| prefLabel |
Southern blot assay
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| term editor |
Marcus Chibucos Philippe Rocca-Serra
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| subClassOf |
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