Preferred Name

enzyme-linked immunospot assay
Synonyms
Definitions

A cytometry assay in which cells are cultured on a surface coated with a capture antibody binding a secretory material of interest which subsequently gets stained resulting in a spot for each cell producing the secretory material of interest.

ID

http://purl.obolibrary.org/obo/OBI_0600031

alternative term

ELISPOT assay

definition

A cytometry assay in which cells are cultured on a surface coated with a capture antibody binding a secretory material of interest which subsequently gets stained resulting in a spot for each cell producing the secretory material of interest.

definition source

IEDB

editor preferred label

enzyme-linked immunospot assay

example of usage

Determination of the frequency of cells producing IFN-gamma in response to viral peptides by placing effector cells on a anti IFN-gamma coated plate, and adding antigen presenting cells pulsed with a peptide. After washing, the bound IFN-gamma is detected with a secondary antibody linked to a dye that visualizes as one spot per cell.

has curation status

http://purl.obolibrary.org/obo/IAO_0000120

label

enzyme-linked immunospot assay

prefixIRI

OBI:0600031

prefLabel

enzyme-linked immunospot assay

term editor

IEDB

subClassOf

http://purl.obolibrary.org/obo/OBI_0001977

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Mapping To Ontology Source
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