An analyte assay that detects specific peptides in an input material by separating it using gel electrophoresis, transfering the separated molecules to a membrane, and staining them with antibodies specific to the analyte molecules.

http://purl.obolibrary.org/obo/OBI_0000854

An analyte assay that detects specific peptides in an input material by separating it using gel electrophoresis, transfering the separated molecules to a membrane, and staining them with antibodies specific to the analyte molecules.

Philippe Rocca-Serra, IEDB

western blot analysis

Running a cell lysate on an acrylamide gel in a western blot aparatus to separate the constituent proteins, followed by transfer of the proteins from the gel to a nitrocellulose membrane. Staining this membrane with specific antibodies to detect the presence of specific proteins of interest.

http://purl.obolibrary.org/obo/IAO_0000120

western blot analysis

OBI:0000854

western blot analysis

Philippe Rocca-Serra, IEDB

http://purl.obolibrary.org/obo/OBI_0001978