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Preferred Name

western blot analysis
Synonyms
Definitions

An analyte assay that detects specific peptides in an input material by separating it using gel electrophoresis, transfering the separated molecules to a membrane, and staining them with antibodies specific to the analyte molecules.
ID

http://purl.obolibrary.org/obo/OBI_0000854
definition

An analyte assay that detects specific peptides in an input material by separating it using gel electrophoresis, transfering the separated molecules to a membrane, and staining them with antibodies specific to the analyte molecules.
definition source

Philippe Rocca-Serra, IEDB
editor preferred label

western blot analysis
example of usage

Running a cell lysate on an acrylamide gel in a western blot aparatus to separate the constituent proteins, followed by transfer of the proteins from the gel to a nitrocellulose membrane. Staining this membrane with specific antibodies to detect the presence of specific proteins of interest.
has curation status

http://purl.obolibrary.org/obo/IAO_0000120
label

western blot analysis
prefixIRI

OBI:0000854
prefLabel

western blot analysis
term editor

Philippe Rocca-Serra, IEDB
subClassOf

http://purl.obolibrary.org/obo/OBI_0001978
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