Preferred Name | western blot analysis | |
Synonyms |
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Definitions |
An analyte assay that detects specific peptides in an input material by separating it using gel electrophoresis, transfering the separated molecules to a membrane, and staining them with antibodies specific to the analyte molecules. |
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ID |
http://purl.obolibrary.org/obo/OBI_0000854 |
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definition |
An analyte assay that detects specific peptides in an input material by separating it using gel electrophoresis, transfering the separated molecules to a membrane, and staining them with antibodies specific to the analyte molecules. |
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definition source |
Philippe Rocca-Serra, IEDB |
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editor preferred label |
western blot analysis |
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example of usage |
Running a cell lysate on an acrylamide gel in a western blot aparatus to separate the constituent proteins, followed by transfer of the proteins from the gel to a nitrocellulose membrane. Staining this membrane with specific antibodies to detect the presence of specific proteins of interest. |
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has curation status | ||
label |
western blot analysis |
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prefixIRI |
OBI:0000854 |
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prefLabel |
western blot analysis |
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term editor |
Philippe Rocca-Serra, IEDB |
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subClassOf |
Delete | Mapping To | Ontology | Source |
---|---|---|---|
http://purl.obolibrary.org/obo/OBI_0000854 | ENM | SAME_URI | |
http://purl.obolibrary.org/obo/OBI_0000854 | PDRO | SAME_URI | |
http://purl.obolibrary.org/obo/OBI_0000854 | OBI | SAME_URI | |
http://purl.obolibrary.org/obo/OBI_0000854 | ONE | SAME_URI | |
http://purl.obolibrary.org/obo/OBI_0000854 | EGO | SAME_URI | |
http://purl.obolibrary.org/obo/OBI_0000854 | BMONT | SAME_URI | |
http://purl.obolibrary.org/obo/OBI_0000854 | ERO | SAME_URI | |
http://purl.obolibrary.org/obo/TEMP#251000000019 | VIMM | LOOM | |
http://purl.obolibrary.org/obo/OBI_0000854 | ONE | LOOM | |
http://purl.obolibrary.org/obo/OBI_0000854 | EGO | LOOM | |
http://purl.obolibrary.org/obo/OBI_0000854 | ERO | LOOM | |
http://purl.bioontology.org/ontology/npo#NPO_1424 | NPO | LOOM |