Ontology for Biomedical Investigations

Last uploaded: October 22, 2024
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Preferred Name

Southern blot assay
Synonyms

Southern blot
Definitions

An analyte assay that detects the presence of a specific sequence in a DNA sample, which has been digested by restriction enzymes, resolved by gel electrophoresis, and blotted to a solid support, followed by hybridization of a probe raised against a specific sequence and detected with fluorescence or radioactivity.
ID

http://purl.obolibrary.org/obo/OBI_0000892
alternative label

Southern blot
Creator

Marcus Chibucos

Philippe Rocca-Serra
definition source

OBI
editor preferred term

Southern blot assay
example of usage

PMID: 9452032. Germline mutations detected in the von Hippel-Lindau disease tumor suppressor gene by Southern blot and direct genomic DNA sequencing.
has curation status

http://purl.obolibrary.org/obo/IAO_0000120
has part

http://purl.obolibrary.org/obo/OBI_0302903
has participant

http://purl.obolibrary.org/obo/IAO_0000109
has_specified_output

http://purl.obolibrary.org/obo/IAO_0000109
label

Southern blot assay
prefixIRI

OBI:0000892
prefLabel

Southern blot assay
term editor

Marcus Chibucos

Philippe Rocca-Serra
textual definition

An analyte assay that detects the presence of a specific sequence in a DNA sample, which has been digested by restriction enzymes, resolved by gel electrophoresis, and blotted to a solid support, followed by hybridization of a probe raised against a specific sequence and detected with fluorescence or radioactivity.
subClassOf

http://purl.obolibrary.org/obo/OBI_0000443
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