loading...| Preferred Name | western blot | |
| Synonyms |
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| Definitions |
A multistep process in which a mixture of proteins is separated by gel electrophoresis, that is, separation of ionic solutes based on differences in rate of migration in an applied electric field using a semisolid colloid such as agarose or acrylamide as the support medium, followed by transfer to a membrane, usually composed of nitrocellulose, nylon or polyvinylidene difluoride (PVDF) and detection of specific proteins by binding of tagged or labelled antibodies. term merged into 'western blot assay' (MMO:0000669) |
|
| ID |
http://purl.obolibrary.org/obo/MMO_0000338 |
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| Obsolete |
true |
|
| comment |
term merged into 'western blot assay' (MMO:0000669) |
|
| created_by |
JSmith |
|
| creation_date |
2013-02-13T17:47:29Z |
|
| definition |
A multistep process in which a mixture of proteins is separated by gel electrophoresis, that is, separation of ionic solutes based on differences in rate of migration in an applied electric field using a semisolid colloid such as agarose or acrylamide as the support medium, followed by transfer to a membrane, usually composed of nitrocellulose, nylon or polyvinylidene difluoride (PVDF) and detection of specific proteins by binding of tagged or labelled antibodies. |
|
| deprecated |
true |
|
| has_obo_namespace |
measurement_method_ontology |
|
| IAO_0000231 | ||
| IAO_0100001 | ||
| id |
MMO:0000338 |
|
| label |
western blot |
|
| notation |
MMO:0000338 |
|
| prefLabel |
western blot |