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Molecular Interactions
Last uploaded:
August 6, 2024
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| Preferred Name | western blot | |
| Synonyms |
Immuno blot |
|
| Definitions |
Western blot is a procedure to identify and quantify proteins. A mixture of protein is first submitted to an electrophoresis in denaturing condition and then electro-transferred from the gel to a membrane. The membrane is then incubated with a primary antibody specific for a given protein or a specific residue modification in the sample under analysis. A secondary antibody, radiolabelled or fused to fluorophore or to a chromogenic enzyme, targets the first antibody and allows the visualisation of the protein band on the membrane. |
|
| ID |
http://purl.obolibrary.org/obo/MI_0113 |
|
| definition |
Western blot is a procedure to identify and quantify proteins. A mixture of protein is first submitted to an electrophoresis in denaturing condition and then electro-transferred from the gel to a membrane. The membrane is then incubated with a primary antibody specific for a given protein or a specific residue modification in the sample under analysis. A secondary antibody, radiolabelled or fused to fluorophore or to a chromogenic enzyme, targets the first antibody and allows the visualisation of the protein band on the membrane.
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| has_exact_synonym |
Immuno blot
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| has_obo_namespace |
PSI-MI
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| id |
MI:0113
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| in_subset | ||
| label |
western blot
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|
| notation |
MI:0113
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| prefLabel |
western blot
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| treeView | ||
| subClassOf |
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