Preferred Name | western blot | |
Synonyms |
Immuno blot |
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Definitions |
Western blot is a procedure to identify and quantify proteins. A mixture of protein is first submitted to an electrophoresis in denaturing condition and then electro-transferred from the gel to a membrane. The membrane is then incubated with a primary antibody specific for a given protein or a specific residue modification in the sample under analysis. A secondary antibody, radiolabelled or fused to fluorophore or to a chromogenic enzyme, targets the first antibody and allows the visualisation of the protein band on the membrane. |
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ID |
http://purl.obolibrary.org/obo/MI_0113 |
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definition |
Western blot is a procedure to identify and quantify proteins. A mixture of protein is first submitted to an electrophoresis in denaturing condition and then electro-transferred from the gel to a membrane. The membrane is then incubated with a primary antibody specific for a given protein or a specific residue modification in the sample under analysis. A secondary antibody, radiolabelled or fused to fluorophore or to a chromogenic enzyme, targets the first antibody and allows the visualisation of the protein band on the membrane. |
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has_exact_synonym |
Immuno blot |
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has_obo_namespace |
PSI-MI |
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id |
MI:0113 |
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in_subset | ||
label |
western blot |
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notation |
MI:0113 |
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prefLabel |
western blot |
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treeView | ||
subClassOf |