mRNA Capping

The 5'-ends of all eukaryotic pre-mRNAs studied thus far are converted to cap structures. The cap is thought to influence splicing of the first intron, and is bound by 'cap-binding' proteins, CBP80 and CBP20, in the nucleus. The cap is important for translation initiation, and it also interacts with the poly(A)terminus, via proteins, resulting in circularization of the mRNA to facilitate multiple rounds of translation. The cap is also important for mRNA stability, protecting it from 5' to 3' nucleases, and is required for mRNA export to the cytoplasm.<BR>The capping reaction usually occurs very rapidly on nascent transcripts; after the synthesis of only a few nucleotides by RNA polymerase II. The capping reaction involves the conversion of the 5'-end of the nascent transcript from a triphosphate to a diphosphate by a RNA 5'-triphosphatase, followed by the addition of a guanosine monophosphate by the mRNA guanylyltransferase, to form a 5'-5'-triphosphate linkage. This cap is then methylated by 2'-O-methyltransferases.<P>

http://purl.obolibrary.org/obo/HINO_0021087

The 5'-ends of all eukaryotic pre-mRNAs studied thus far are converted to cap structures. The cap is thought to influence splicing of the first intron, and is bound by 'cap-binding' proteins, CBP80 and CBP20, in the nucleus. The cap is important for translation initiation, and it also interacts with the poly(A)terminus, via proteins, resulting in circularization of the mRNA to facilitate multiple rounds of translation. The cap is also important for mRNA stability, protecting it from 5' to 3' nucleases, and is required for mRNA export to the cytoplasm.
The capping reaction usually occurs very rapidly on nascent transcripts; after the synthesis of only a few nucleotides by RNA polymerase II. The capping reaction involves the conversion of the 5'-end of the nascent transcript from a triphosphate to a diphosphate by a RNA 5'-triphosphatase, followed by the addition of a guanosine monophosphate by the mRNA guanylyltransferase, to form a 5'-5'-triphosphate linkage. This cap is then methylated by 2'-O-methyltransferases.

Pubmed10395561

Pubmed11909521

Pubmed3326038

Reactome, http://www.reactome.org

Pubmed11017188

mRNA Capping

http://purl.obolibrary.org/obo/NCBITaxon_9606

HINO:0021087

mRNA Capping

ReactomeREACT_1470

Reactome Database ID Release 4372086

GENE ONTOLOGYGO:0006370

http://purl.obolibrary.org/obo/INO_0000021

http://purl.obolibrary.org/obo/HINO_0009045

http://purl.obolibrary.org/obo/HINO_0009046

http://purl.obolibrary.org/obo/HINO_0009044

http://purl.obolibrary.org/obo/HINO_0009049

http://purl.obolibrary.org/obo/HINO_0009048

http://purl.obolibrary.org/obo/HINO_0009047

http://purl.obolibrary.org/obo/HINO_0009051

http://purl.obolibrary.org/obo/HINO_0009050

http://purl.obolibrary.org/obo/HINO_0019834

http://purl.obolibrary.org/obo/HINO_0019837

http://purl.obolibrary.org/obo/HINO_0019836