loading...| Preferred Name |
Synthesis of glycosylphosphatidylinositol (GPI) |
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| Synonyms |
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| Definitions |
Reviewed: Orlean, P, 0000-00-00 00:00:00 Glycosylphosphatidyl inositol (GPI) acts as a membrane anchor for many cell surface proteins. GPI is synthesized in the endoplasmic reticulum. In humans, a single pathway consisting of nine reactions appears to be responsible for the synthesis of the major GPI species involved in membrane protein anchoring. This pathway is shown in the figure. Two additional reactions, not shown, allow the synthesis of variant forms of GPI, one with an additional mannose residue and one with an additional ethanolamine (Tauron et al. 2004; Shishioh et al. 2005). These variant GPI molecules may be used for tissue-specific protein modifications, or may function independently.<p>The steps of GPI synthesis were first identified by isolating large numbers of mutant cell lines that had lost the ability to express GPI anchored proteins on their surfaces. Somatic cell hybrid analyses of these lines allowed the definition of complementation groups corresponding to distinct mutated genes, and cDNAs corresponding to normal forms of these genes were identified on the basis of their abilities to restore normal cell surface protein expression in mutant cells. Co-precipitation experiments with tagged cloned proteins have allowed the identification of additional proteins involved in GPI anchor biosynthesis. Authored: D'Eustachio, P, 2005-04-04 21:01:34 Edited: D'Eustachio, P, 0000-00-00 00:00:00 |
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| ID |
http://purl.obolibrary.org/obo/HINO_0016720 |
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| comment |
Reviewed: Orlean, P, 0000-00-00 00:00:00 Glycosylphosphatidyl inositol (GPI) acts as a membrane anchor for many cell surface proteins. GPI is synthesized in the endoplasmic reticulum. In humans, a single pathway consisting of nine reactions appears to be responsible for the synthesis of the major GPI species involved in membrane protein anchoring. This pathway is shown in the figure. Two additional reactions, not shown, allow the synthesis of variant forms of GPI, one with an additional mannose residue and one with an additional ethanolamine (Tauron et al. 2004; Shishioh et al. 2005). These variant GPI molecules may be used for tissue-specific protein modifications, or may function independently. The steps of GPI synthesis were first identified by isolating large numbers of mutant cell lines that had lost the ability to express GPI anchored proteins on their surfaces. Somatic cell hybrid analyses of these lines allowed the definition of complementation groups corresponding to distinct mutated genes, and cDNAs corresponding to normal forms of these genes were identified on the basis of their abilities to restore normal cell surface protein expression in mutant cells. Co-precipitation experiments with tagged cloned proteins have allowed the identification of additional proteins involved in GPI anchor biosynthesis. Authored: D'Eustachio, P, 2005-04-04 21:01:34 Edited: D'Eustachio, P, 0000-00-00 00:00:00 |
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| definition source |
Reactome, http://www.reactome.org Pubmed11102867 Pubmed15632136 Pubmed15208306 |
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| label |
Synthesis of glycosylphosphatidylinositol (GPI) |
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| prefixIRI |
HINO:0016720 |
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| prefLabel |
Synthesis of glycosylphosphatidylinositol (GPI) |
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| seeAlso |
ReactomeREACT_952 Reactome Database ID Release 43162710 GENE ONTOLOGYGO:0016254 |
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| has_part |
http://purl.obolibrary.org/obo/HINO_0025928 http://purl.obolibrary.org/obo/HINO_0025926 http://purl.obolibrary.org/obo/HINO_0025927 http://purl.obolibrary.org/obo/HINO_0025929 http://purl.obolibrary.org/obo/HINO_0025930 http://purl.obolibrary.org/obo/HINO_0025933 http://purl.obolibrary.org/obo/HINO_0025941 http://purl.obolibrary.org/obo/HINO_0025942 http://purl.obolibrary.org/obo/HINO_0025944 |