Preferred Name | ELISPOT assay | |
Synonyms |
ELISPOT Enzyme-linked immunosorbent spot assay |
|
Definitions |
An assay in which the number of cells producing a material in a larger cell population are stained and counted. This is done by visualizing and counting the number of spots present on a surface via an antibody specific for the produced material. Each spot represents one cell producing the material of interest. |
|
ID |
http://purl.obolibrary.org/obo/OBI_0600031 |
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alternative term |
ELISPOT Enzyme-linked immunosorbent spot assay |
|
definition |
An assay in which the number of cells producing a material in a larger cell population are stained and counted. This is done by visualizing and counting the number of spots present on a surface via an antibody specific for the produced material. Each spot represents one cell producing the material of interest. |
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definition source |
IEDB |
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editor note |
2/26/09: RESOLVE: we need to specify the produced substance that is desired to be detected. Analyte does not work, as this is not a concentration, and this is not present at the start of the assay. |
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editor preferred term |
ELISPOT assay |
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example of usage |
Determination of the frequency of cells producing IFN-gamma in response to viral peptides by placing effector cells on a anti IFN-gamma coated plate, and adding antigen presenting cells pulsed with a peptide. After washing, the bound IFN-gamma is detected with a secondary antibody linked to a dye that visualizes as one spot per cell. |
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has curation status | ||
imported from | ||
label |
ELISPOT assay |
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prefixIRI |
OBI:0600031 |
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prefLabel |
ELISPOT assay |
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term editor |
IEDB |
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subClassOf |
Delete | Mapping To | Ontology | Source |
---|---|---|---|
http://purl.obolibrary.org/obo/OBI_0600031 | ENM | SAME_URI | |
http://purl.obolibrary.org/obo/OBI_0600031 | PDRO | SAME_URI | |
http://purl.obolibrary.org/obo/OBI_0600031 | OBI | SAME_URI | |
http://purl.obolibrary.org/obo/OBI_0600031 | ONE | SAME_URI | |
http://purl.obolibrary.org/obo/OBI_0600031 | EGO | SAME_URI | |
http://purl.obolibrary.org/obo/OBI_0600031 | OBI_IEE | SAME_URI | |
http://purl.jp/bio/4/id/200906062738618671 | IOBC | LOOM |