Eagle-I Research Resource Ontology

Last uploaded: January 14, 2016
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Preferred Name

ELISPOT assay
Synonyms

ELISPOT

Enzyme-linked immunosorbent spot assay
Definitions

An assay in which the number of cells producing a material in a larger cell population are stained and counted. This is done by visualizing and counting the number of spots present on a surface via an antibody specific for the produced material. Each spot represents one cell producing the material of interest.
ID

http://purl.obolibrary.org/obo/OBI_0600031
alternative term

ELISPOT

Enzyme-linked immunosorbent spot assay
definition

An assay in which the number of cells producing a material in a larger cell population are stained and counted. This is done by visualizing and counting the number of spots present on a surface via an antibody specific for the produced material. Each spot represents one cell producing the material of interest.
definition source

IEDB
editor note

2/26/09: RESOLVE: we need to specify the produced substance that is desired to be detected. Analyte does not work, as this is not a concentration, and this is not present at the start of the assay.
editor preferred term

ELISPOT assay
example of usage

Determination of the frequency of cells producing IFN-gamma in response to viral peptides by placing effector cells on a anti IFN-gamma coated plate, and adding antigen presenting cells pulsed with a peptide. After washing, the bound IFN-gamma is detected with a secondary antibody linked to a dye that visualizes as one spot per cell.
has curation status

http://purl.obolibrary.org/obo/IAO_0000125
imported from

http://purl.obolibrary.org/obo/obi.owl
label

ELISPOT assay
prefixIRI

OBI:0600031
prefLabel

ELISPOT assay
term editor

IEDB
subClassOf

http://purl.obolibrary.org/obo/ERO_0000401
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