Eagle-I Research Resource Ontology

Last uploaded: January 14, 2016
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Preferred Name

Southern blot analysis
Synonyms

Southern blot
Definitions

Southern blot analysis is a an assay used in molecular biology to assert the presence/absence status of a specific DNA sequence in DNA samples. DNA samples to be assayed are first digested by restriction enzymes, fragments are then resolved by gel electrophoresis following by a blotting ensuring transfer to nitrocellulose or nylon membrane. Immobilization of DNA fragments to the membrane is achieved by UV crosslinking and/or baking. Probes raised against the specific sequences are then hybridized to the membrane. Detection of hybridization signals is carried out by immunofluorescence or radioactivity measurements using photographic films or digital imaging devices such as Phosphor Imager.
ID

http://purl.obolibrary.org/obo/OBI_0000892
alternative term

Southern blot
definition

Southern blot analysis is a an assay used in molecular biology to assert the presence/absence status of a specific DNA sequence in DNA samples. DNA samples to be assayed are first digested by restriction enzymes, fragments are then resolved by gel electrophoresis following by a blotting ensuring transfer to nitrocellulose or nylon membrane. Immobilization of DNA fragments to the membrane is achieved by UV crosslinking and/or baking. Probes raised against the specific sequences are then hybridized to the membrane. Detection of hybridization signals is carried out by immunofluorescence or radioactivity measurements using photographic films or digital imaging devices such as Phosphor Imager.
definition source

OBI & Wikipedia
editor preferred term

Southern blot analysis
example of usage

PMID: 9452032. Germline mutations detected in the von Hippel-Lindau disease tumor suppressor gene by Southern blot and direct genomic DNA sequencing. Li C, Weber G, Ekman P, Lagercrantz J, Norlen BJ, Akerström G, Nordenskjöld M, Bergerheim US. Hum Mutat. 1998;Suppl 1:S31-3.
has curation status

http://purl.obolibrary.org/obo/IAO_0000120
imported from

http://purl.obolibrary.org/obo/obi.owl
label

Southern blot analysis
prefixIRI

OBI:0000892
prefLabel

Southern blot analysis
term editor

Person: Philippe Rocca-Serra
subClassOf

http://purl.obolibrary.org/obo/ERO_0001286
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