eNanoMapper

Last uploaded: July 12, 2023
Preferred Name

western blot assay
Synonyms
Definitions

An analyte assay that detects specific peptides in an input material by separating it using gel electrophoresis, transfering the separated molecules to a membrane, and staining them with_ antibodies specific to the analyte molecules.

ID

http://purl.obolibrary.org/obo/OBI_0000854

label

western blot assay

prefixIRI

OBI:0000854

prefLabel

western blot assay

term editor

Philippe Rocca-Serra

IEDB

定义来源

Philippe Rocca-Serra

IEDB

文本定义

An analyte assay that detects specific peptides in an input material by separating it using gel electrophoresis, transfering the separated molecules to a membrane, and staining them with_ antibodies specific to the analyte molecules.

有管理状态

http://purl.obolibrary.org/obo/IAO_0000120

用法示例

Running a cell lysate on an acrylamide gel in a western blot aparatus to separate the constituent proteins, followed by transfer of the proteins from the gel to a nitrocellulose membrane. Staining this membrane with specific antibodies to detect the presence of specific proteins of interest.

编辑首选术语~编辑首选标签

western blot assay

subClassOf

http://purl.obolibrary.org/obo/OBI_0001978

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