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Epigenome Ontology
Last uploaded:
July 3, 2016
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| Preferred Name | enzyme-linked immunospot assay | |
| Synonyms |
ELISPOT assay |
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| Definitions |
A cytometry assay in which cells are cultured on a surface coated with a capture antibody binding a secretory material of interest which subsequently gets stained resulting in a spot for each cell producing the secretory material of interest. |
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| ID |
http://purl.obolibrary.org/obo/OBI_0600031 |
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| alternative term |
ELISPOT assay
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|
| definition |
A cytometry assay in which cells are cultured on a surface coated with a capture antibody binding a secretory material of interest which subsequently gets stained resulting in a spot for each cell producing the secretory material of interest.
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| definition source |
IEDB
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| editor preferred term |
enzyme-linked immunospot assay
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| example of usage |
Determination of the frequency of cells producing IFN-gamma in response to viral peptides by placing effector cells on a anti IFN-gamma coated plate, and adding antigen presenting cells pulsed with a peptide. After washing, the bound IFN-gamma is detected with a secondary antibody linked to a dye that visualizes as one spot per cell.
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| has curation status | ||
| label |
enzyme-linked immunospot assay
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| prefixIRI |
OBI:0600031
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| prefLabel |
enzyme-linked immunospot assay
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|
| term editor |
IEDB
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| subClassOf |
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