Preferred Name | enzyme-linked immunospot assay | |
Synonyms |
ELISPOT assay |
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Definitions |
A cytometry assay in which cells are cultured on a surface coated with a capture antibody binding a secretory material of interest which subsequently gets stained resulting in a spot for each cell producing the secretory material of interest. |
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ID |
http://purl.obolibrary.org/obo/OBI_0600031 |
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alternative term |
ELISPOT assay |
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definition |
A cytometry assay in which cells are cultured on a surface coated with a capture antibody binding a secretory material of interest which subsequently gets stained resulting in a spot for each cell producing the secretory material of interest. |
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definition source |
IEDB |
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editor preferred term |
enzyme-linked immunospot assay |
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example of usage |
Determination of the frequency of cells producing IFN-gamma in response to viral peptides by placing effector cells on a anti IFN-gamma coated plate, and adding antigen presenting cells pulsed with a peptide. After washing, the bound IFN-gamma is detected with a secondary antibody linked to a dye that visualizes as one spot per cell. |
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has curation status | ||
label |
enzyme-linked immunospot assay |
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prefixIRI |
OBI:0600031 |
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prefLabel |
enzyme-linked immunospot assay |
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term editor |
IEDB |
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subClassOf |