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Epigenome Ontology
Last uploaded:
July 3, 2016
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| Preferred Name | western blot analysis | |
| Synonyms |
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| Definitions |
An analyte assay that detects specific peptides in an input material by separating it using gel electrophoresis, transfering the separated molecules to a membrane, and staining them with antibodies specific to the analyte molecules. |
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| ID |
http://purl.obolibrary.org/obo/OBI_0000854 |
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| definition |
An analyte assay that detects specific peptides in an input material by separating it using gel electrophoresis, transfering the separated molecules to a membrane, and staining them with antibodies specific to the analyte molecules.
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| definition source |
Philippe Rocca-Serra, IEDB
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| editor preferred term |
western blot analysis
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| example of usage |
Running a cell lysate on an acrylamide gel in a western blot aparatus to separate the constituent proteins, followed by transfer of the proteins from the gel to a nitrocellulose membrane. Staining this membrane with specific antibodies to detect the presence of specific proteins of interest.
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| has curation status | ||
| label |
western blot analysis
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| prefixIRI |
OBI:0000854
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| prefLabel |
western blot analysis
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| term editor |
Philippe Rocca-Serra, IEDB
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| subClassOf |
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