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Bone and Cartilage Tissue Engineering Ontology
Last uploaded:
June 18, 2014
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| Preferred Name | Western blot | |
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| Definitions |
Procedure to identify and quantify proteins. A mixture of protein is first submitted to an electrophoresis in denaturing condition and then electro-transferred from the gel to a membrane. The membrane is then incubated with a primary antibody specific for a given protein or a specific residue modification in the sample under analysis. A secondary antibody, radiolabelled or fused to fluorophore or to a chromogenic enzyme, targets the first antibody and allows the visualisation of the protein band on the membrane. |
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| ID |
http://purl.obolibrary.org/obo/ID_0000161 |
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| definition |
Procedure to identify and quantify proteins. A mixture of protein is first submitted to an electrophoresis in denaturing condition and then electro-transferred from the gel to a membrane. The membrane is then incubated with a primary antibody specific for a given protein or a specific residue modification in the sample under analysis. A secondary antibody, radiolabelled or fused to fluorophore or to a chromogenic enzyme, targets the first antibody and allows the visualisation of the protein band on the membrane.
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| has_obo_namespace |
mi
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| id |
ID:0000161
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| label |
Western blot
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| notation |
ID:0000161
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| prefLabel |
Western blot
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| subClassOf |
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