Bioluminescence resonance energy transfer

90.60.110.10.10^Bioluminescence resonance energy transfer|90.200.10.10^Bioluminescence resonance energy transfer

Bioluminescence resonance energy transfer

BRET assay technology is based on the efficient Resonance Energy Transfer (RET) between a bioluminescent donor moiety and a fluorescent acceptor moiety. RET was first described in the 1940’s by Förster and is characterized by the radiationless transfer of excited state energy from a donor to an acceptor molecule. From a practical point of view, Förster and others have shown that energy transfer efficiency is highly dependent on the distance between the donor and acceptor moieties and their relative orientation with respect to each other. In most RET-based assays, the typical effective distance between the donor and acceptor is 10 to 100 angstroms (Å). This range correlates well with most biological interactions thus making RET an excellent tool for monitoring macromolecular interac- tions. Examples of RET-based technologies are Fluorescence Resonance Energy Transfer (FRET) and Bioluminescence Resonance Energy Transfer (BRET). http://www2.cbm.uam.es/confocal/Manuales/bret.pdf RD

Accepted