National Cancer Institute Thesaurus

Last uploaded: February 23, 2024
Preferred Name

Immunohistochemistry Staining Method
Synonyms

Immunohistochemistry Staining Method

Immunohistochemistry (IHC)

immunohistochemistry

Immunohistochemistry

Cell/Tissue, Immunohistochemistry

IHC

Definitions

Immunohistochemical staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.

ID

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C23020

ALT_DEFINITION

Immunohistochemical staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.

A technique used to identify specific molecules in different kinds of tissue. The tissue is treated with antibodies that bind the specific molecule. These are made visible under a microscope by using a color reaction, a radioisotope, colloidal gold, or a fluorescent dye. Immunohistochemistry is used to help diagnose diseases, such as cancer, and to detect the presence of microorganisms. It is also used in basic research to understand how cells grow and differentiate (become more specialized).

code

C23020

Concept_In_Subset

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C66830

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C157711

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C61410

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C116977

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C118169

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C85492

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C177537

Contributing_Source

CDISC

CTRP

GDC

DEFINITION

Immunohistochemical staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.

Display_Name

Immunohistochemistry Staining Method

FULL_SYN

Immunohistochemistry Staining Method

Immunohistochemistry (IHC)

immunohistochemistry

Immunohistochemistry

Cell/Tissue, Immunohistochemistry

IHC

Is_Value_For_GDC_Property

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C19770

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C23010

label

Immunohistochemistry Staining Method

Legacy Concept Name

Immunohistochemistry_Staining_Method

Maps_To

Immunohistochemistry (IHC)

IHC

Preferred_Name

Immunohistochemistry Staining Method

prefixIRI

Thesaurus:C23020

Semantic_Type

Laboratory Procedure

UMLS_CUI

C0021044

subClassOf

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C23044

Delete Subject Author Type Created
No notes to display