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Measurement Method Ontology
Last uploaded:
July 10, 2023
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| Preferred Name | Southern blot | |
| Synonyms |
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| Definitions |
A multistep process in which a mixture of DNA (deoxyribonucleic acid) is separated by gel electrophoresis, that is, separation of ionic solutes based on differences in rate of migration in an applied electric field using a semisolid colloid such as agarose or acrylamide as the support medium, followed by transfer to a membrane such as nitrocellulose or nylon and detection of specific RNAs by hybridization of a tagged or labelled polynucleotide, usually a single DNA fragment with a specific, known sequence. |
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| ID |
http://purl.obolibrary.org/obo/MMO_0000340 |
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| created_by |
JSmith
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| creation_date |
2013-02-13T17:59:29Z
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| definition |
A multistep process in which a mixture of DNA (deoxyribonucleic acid) is separated by gel electrophoresis, that is, separation of ionic solutes based on differences in rate of migration in an applied electric field using a semisolid colloid such as agarose or acrylamide as the support medium, followed by transfer to a membrane such as nitrocellulose or nylon and detection of specific RNAs by hybridization of a tagged or labelled polynucleotide, usually a single DNA fragment with a specific, known sequence.
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| has_obo_namespace |
measurement_method_ontology
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| id |
MMO:0000340
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| label |
Southern blot
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| notation |
MMO:0000340
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| prefLabel |
Southern blot
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| treeView | ||
| subClassOf |
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