Preferred Name | Southern blot | |
Synonyms |
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Definitions |
A multistep process in which a mixture of DNA (deoxyribonucleic acid) is separated by gel electrophoresis, that is, separation of ionic solutes based on differences in rate of migration in an applied electric field using a semisolid colloid such as agarose or acrylamide as the support medium, followed by transfer to a membrane such as nitrocellulose or nylon and detection of specific RNAs by hybridization of a tagged or labelled polynucleotide, usually a single DNA fragment with a specific, known sequence. |
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ID |
http://purl.obolibrary.org/obo/MMO_0000340 |
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created_by |
JSmith |
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creation_date |
2013-02-13T17:59:29Z |
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definition |
A multistep process in which a mixture of DNA (deoxyribonucleic acid) is separated by gel electrophoresis, that is, separation of ionic solutes based on differences in rate of migration in an applied electric field using a semisolid colloid such as agarose or acrylamide as the support medium, followed by transfer to a membrane such as nitrocellulose or nylon and detection of specific RNAs by hybridization of a tagged or labelled polynucleotide, usually a single DNA fragment with a specific, known sequence. |
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has_obo_namespace |
measurement_method_ontology |
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id |
MMO:0000340 |
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label |
Southern blot |
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notation |
MMO:0000340 |
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prefLabel |
Southern blot |
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treeView | ||
subClassOf |