Measurement Method Ontology

Last uploaded: July 10, 2023
Preferred Name

Southern blot
Synonyms
Definitions

A multistep process in which a mixture of DNA (deoxyribonucleic acid) is separated by gel electrophoresis, that is, separation of ionic solutes based on differences in rate of migration in an applied electric field using a semisolid colloid such as agarose or acrylamide as the support medium, followed by transfer to a membrane such as nitrocellulose or nylon and detection of specific RNAs by hybridization of a tagged or labelled polynucleotide, usually a single DNA fragment with a specific, known sequence.

ID

http://purl.obolibrary.org/obo/MMO_0000340

created_by

JSmith

creation_date

2013-02-13T17:59:29Z

definition

A multistep process in which a mixture of DNA (deoxyribonucleic acid) is separated by gel electrophoresis, that is, separation of ionic solutes based on differences in rate of migration in an applied electric field using a semisolid colloid such as agarose or acrylamide as the support medium, followed by transfer to a membrane such as nitrocellulose or nylon and detection of specific RNAs by hybridization of a tagged or labelled polynucleotide, usually a single DNA fragment with a specific, known sequence.

has_obo_namespace

measurement_method_ontology

id

MMO:0000340

label

Southern blot

notation

MMO:0000340

prefLabel

Southern blot

treeView

http://purl.obolibrary.org/obo/MMO_0000100

subClassOf

http://purl.obolibrary.org/obo/MMO_0000100

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