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Molecular Interactions
Last uploaded:
August 6, 2024
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Preferred Name | clip | |
Synonyms |
cross linking immunoprecipitation cross-linking immunoprecipitation clip CLIP |
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Definitions |
Combination of cross-linking and co-immunoprecipitation aimed to find protein-RNA interactions. The canonical method uses first a cross-linking procedure over a tissue sample or lysate, and the immunoprecipitated with specific antibodies for the protein of interest. Unspecific proteins are digested via proteinase K treatment. RNA can be then identified via Northern blotting or using RT-PCR and then sequencing of the generated cDNA. https://en.wikipedia.org/wiki/CLIP |
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ID |
http://purl.obolibrary.org/obo/MI_2191 |
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created_by |
ppm
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creation_date |
2015-06-15T10:17:34Z
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definition |
Combination of cross-linking and co-immunoprecipitation aimed to find protein-RNA interactions. The canonical method uses first a cross-linking procedure over a tissue sample or lysate, and the immunoprecipitated with specific antibodies for the protein of interest. Unspecific proteins are digested via proteinase K treatment. RNA can be then identified via Northern blotting or using RT-PCR and then sequencing of the generated cDNA.
https://en.wikipedia.org/wiki/CLIP
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has_exact_synonym |
cross linking immunoprecipitation cross-linking immunoprecipitation clip CLIP
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has_obo_namespace |
PSI-MI
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id |
MI:2191
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in_subset | ||
label |
clip
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notation |
MI:2191
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prefLabel |
clip
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treeView | ||
subClassOf |
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