Molecular Interactions

Last uploaded: August 6, 2024
Preferred Name

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Synonyms

cross linking immunoprecipitation

cross-linking immunoprecipitation

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CLIP

Definitions

Combination of cross-linking and co-immunoprecipitation aimed to find protein-RNA interactions. The canonical method uses first a cross-linking procedure over a tissue sample or lysate, and the immunoprecipitated with specific antibodies for the protein of interest. Unspecific proteins are digested via proteinase K treatment. RNA can be then identified via Northern blotting or using RT-PCR and then sequencing of the generated cDNA. https://en.wikipedia.org/wiki/CLIP

ID

http://purl.obolibrary.org/obo/MI_2191

created_by

ppm

creation_date

2015-06-15T10:17:34Z

definition

Combination of cross-linking and co-immunoprecipitation aimed to find protein-RNA interactions. The canonical method uses first a cross-linking procedure over a tissue sample or lysate, and the immunoprecipitated with specific antibodies for the protein of interest. Unspecific proteins are digested via proteinase K treatment. RNA can be then identified via Northern blotting or using RT-PCR and then sequencing of the generated cDNA. https://en.wikipedia.org/wiki/CLIP

has_exact_synonym

cross linking immunoprecipitation

cross-linking immunoprecipitation

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CLIP

has_obo_namespace

PSI-MI

id

MI:2191

in_subset

http://purl.obolibrary.org/obo/mi#PSI-MI_slim

label

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notation

MI:2191

prefLabel

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treeView

http://purl.obolibrary.org/obo/MI_0430

subClassOf

http://purl.obolibrary.org/obo/MI_0430

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