Cells exposed to castanospermine or 1-deoxynojirimycin (inhibitors of the glucosidase enzymes GCS1 and GANAB), are still able to carry out glycosylation and produce complex glycans. This is due to the existence of an alternative route catalyzed by the enzyme endomannosidase (Moore and Spiro, 1990).<br>Glycoproteins that pass through this route probably skip or have a reduced interaction with the Calnexin/Calreticulin cycle, and are transported to the cis-golgi through a route that has not been described yet (probably through the general ER to Golgi flow). Here, the Endomannosidase enzyme, which resides on the Golgi membrane (Hardt et al 2005; Hamilton et al 2005) is able to remove the tri-, di-, or mono-glucose substituted mannose on branch A, leading to a deglucosylated N-glycan structure (Lubas and Spiro, 1988). Edited: Jassal, B, 2010-09-17 has a Stoichiometric coefficient of 3 Reviewed: Gagneux, P, 2010-11-18 Authored: Dall'Olio, GM, 2009-11-10

http://purl.obolibrary.org/obo/HINO_0025595

Cells exposed to castanospermine or 1-deoxynojirimycin (inhibitors of the glucosidase enzymes GCS1 and GANAB), are still able to carry out glycosylation and produce complex glycans. This is due to the existence of an alternative route catalyzed by the enzyme endomannosidase (Moore and Spiro, 1990).
Glycoproteins that pass through this route probably skip or have a reduced interaction with the Calnexin/Calreticulin cycle, and are transported to the cis-golgi through a route that has not been described yet (probably through the general ER to Golgi flow). Here, the Endomannosidase enzyme, which resides on the Golgi membrane (Hardt et al 2005; Hamilton et al 2005) is able to remove the tri-, di-, or mono-glucose substituted mannose on branch A, leading to a deglucosylated N-glycan structure (Lubas and Spiro, 1988).

Edited: Jassal, B, 2010-09-17

has a Stoichiometric coefficient of 3

Reviewed: Gagneux, P, 2010-11-18

Authored: Dall'Olio, GM, 2009-11-10

Pubmed15760709

Reactome, http://www.reactome.org

Pubmed3346233

Pubmed2165493

Pubmed15677381

http://purl.obolibrary.org/obo/CHEBI_59084

http://purl.obolibrary.org/obo/CHEBI_17925

http://purl.obolibrary.org/obo/CHEBI_59089

Alternative endo-mannosidase I route

HINO:0025595

Alternative endo-mannosidase I route

Reactome Database ID Release 43964759

ReactomeREACT_25136

EC Number: 3.2.1.130

http://purl.obolibrary.org/obo/INO_0000040