Proteins that are released from the CNX or CRT complex with folding defects accumulate in a compartment of the ER called ERQC (Kamhi-Nesher S et al 2001). Here, the enzymes UGGG1 or UGGG2 are able to recognize glycoproteins with minor folding process and re-add the glucose on the alpha,1,3 branch; this is a signal for the transport of these glycoproteins back to the ER, where they can interact again with CNX or CRT in order to achieve a correct folding. At the same time that the glycoprotein is in the ERQC, the enzyme ER mannosidase I progressively removes the mannoses in position 1A, 2A, B, C on the N-glycans; when the mannose on 1A is trimmed, UGGG1/UGGG2 is no longer able to re-add the glucose, and therefore the protein is destined for degradation.<br>For years it has been thought that the removal of the mannose in position B of the N-glycan was the signal to direct proteins to degradation. However, this mechanism has been described better by Avezov et al (Avezov V et al, 2008) and it has been demonstrated that even glycoproteins with Man8 or Man7 glycans can be re-glucosylated and interact again with CNX or CRT. For a review on this topic, see Lederkremer GZ, 2009 and Määttänen P et al, 2010. Edited: Jassal, B, 2010-07-02 Reviewed: Gagneux, P, 2010-11-18 Authored: Dall'Olio, GM, 2009-11-10

http://purl.obolibrary.org/obo/HINO_0016800

Proteins that are released from the CNX or CRT complex with folding defects accumulate in a compartment of the ER called ERQC (Kamhi-Nesher S et al 2001). Here, the enzymes UGGG1 or UGGG2 are able to recognize glycoproteins with minor folding process and re-add the glucose on the alpha,1,3 branch; this is a signal for the transport of these glycoproteins back to the ER, where they can interact again with CNX or CRT in order to achieve a correct folding. At the same time that the glycoprotein is in the ERQC, the enzyme ER mannosidase I progressively removes the mannoses in position 1A, 2A, B, C on the N-glycans; when the mannose on 1A is trimmed, UGGG1/UGGG2 is no longer able to re-add the glucose, and therefore the protein is destined for degradation.
For years it has been thought that the removal of the mannose in position B of the N-glycan was the signal to direct proteins to degradation. However, this mechanism has been described better by Avezov et al (Avezov V et al, 2008) and it has been demonstrated that even glycoproteins with Man8 or Man7 glycans can be re-glucosylated and interact again with CNX or CRT. For a review on this topic, see Lederkremer GZ, 2009 and Määttänen P et al, 2010.

Edited: Jassal, B, 2010-07-02

Reviewed: Gagneux, P, 2010-11-18

Authored: Dall'Olio, GM, 2009-11-10

Reactome, http://www.reactome.org

Pubmed11408579

Pubmed19616933

Pubmed18003979

Pubmed20347046

ER Quality Control Compartment (ERQC)

http://purl.obolibrary.org/obo/NCBITaxon_9606

HINO:0016800

ER Quality Control Compartment (ERQC)

Reactome Database ID Release 43901032

ReactomeREACT_25091

http://purl.obolibrary.org/obo/INO_0000021

http://purl.obolibrary.org/obo/HINO_0025364

http://purl.obolibrary.org/obo/HINO_0025365

http://purl.obolibrary.org/obo/HINO_0025366

http://purl.obolibrary.org/obo/HINO_0025367

http://purl.obolibrary.org/obo/HINO_0025368

http://purl.obolibrary.org/obo/HINO_0025370

http://purl.obolibrary.org/obo/HINO_0025372