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Experimental Factor Ontology
Last uploaded:
January 16, 2025
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| Preferred Name | fluorescence-activated cell sorting | |
| Synonyms |
FAC sorting FACS |
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| Definitions |
A flow cytometry assay that provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell. The cells are suspended in a stream of fluid and forced individually through a vibrating nozzle, then exposed to a laser beam and the resulting fluorescence and scattered light is detected. Finally the cells are sorted by applying an electrical charge to droplets of the fluid and deflecting it to the left or right using charged electrodes. |
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| ID |
http://www.ebi.ac.uk/efo/EFO_0009108 |
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| database_cross_reference |
ERO:0000720 NCIt:C17353
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| definition |
A flow cytometry assay that provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell.
The cells are suspended in a stream of fluid and forced individually through a vibrating nozzle, then exposed to a laser beam and the resulting fluorescence and scattered light is detected. Finally the cells are sorted by applying an electrical charge to droplets of the fluid and deflecting it to the left or right using charged electrodes.
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| has_exact_synonym |
FAC sorting FACS
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| label |
fluorescence-activated cell sorting
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| preferred label |
fluorescence-activated cell sorting
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| prefixIRI |
efo:EFO_0009108
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| prefLabel |
fluorescence-activated cell sorting
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| term editor |
Dani Welter
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| subClassOf |
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