Experimental Factor Ontology

Last uploaded: October 15, 2024
Preferred Name

fluorescence-activated cell sorting
Synonyms

FAC sorting

FACS

Definitions

A flow cytometry assay that provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell. The cells are suspended in a stream of fluid and forced individually through a vibrating nozzle, then exposed to a laser beam and the resulting fluorescence and scattered light is detected. Finally the cells are sorted by applying an electrical charge to droplets of the fluid and deflecting it to the left or right using charged electrodes.

ID

http://www.ebi.ac.uk/efo/EFO_0009108

database_cross_reference

ERO:0000720

NCIt:C17353

definition

A flow cytometry assay that provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell. The cells are suspended in a stream of fluid and forced individually through a vibrating nozzle, then exposed to a laser beam and the resulting fluorescence and scattered light is detected. Finally the cells are sorted by applying an electrical charge to droplets of the fluid and deflecting it to the left or right using charged electrodes.

has_exact_synonym

FAC sorting

FACS

label

fluorescence-activated cell sorting

preferred label

fluorescence-activated cell sorting

prefixIRI

efo:EFO_0009108

prefLabel

fluorescence-activated cell sorting

term editor

Dani Welter

subClassOf

http://purl.obolibrary.org/obo/OBI_0000512

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