Cigarette Smoke Exposure Ontology

Last uploaded: July 10, 2014
Preferred Name

Polyacrylamide Gel Electrophoresis
Synonyms
ID

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#Polyacrylamide_Gel_Electrophoresis

DEFINITION

Analytical and separative procedures in which molecules, particularly proteins or nucleic acids, are separated by their different electrophoretic mobilities in a hydrated gel. The gel suppresses convective mixing of the fluid phase through which the electrophoresis takes place and contributes molecular sieving. For proteins, commonly carried out in the presence of the anionic detergent sodium dodecylsulphate (SDS). SDS denatures proteins so that noncovalently associating sub unit polypeptides migrate independently and by binding to the proteins confers a net negative charge roughly proportional to the chain weight.

FULL_SYN

PAGE

Electrophoresis, Polyacrylamide Gel

Fractionation, Polyacrylamide Gel

label

Polyacrylamide Gel Electrophoresis

prefixIRI

Thesaurus:Polyacrylamide_Gel_Electrophoresis

prefLabel

Polyacrylamide Gel Electrophoresis

subClassOf

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#Gel_Electrophoresis

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