BioAssay Ontology

Last uploaded: February 21, 2024
Preferred Name

cell line
Synonyms
Definitions

A cultured cell population that represents a genetically stable and homogenous population of cultured cells that shares a common propagation history (i.e. has been successively passaged together in culture). A cultured cell population that represents a genetically stable and homogenous population of cultured cells that shares a common propagation history (i.e. has been successively passaged together in culture). The term 'line' is used when a culture has undergone an intentional experimental process to establish a more uniform and stable population of cells (see 'establishing cell line'). This will require one or more passages, but may involve additional selection processes. Through such passaging and/or selection processes, the resulting 'line' attains some level of genetic stability and compositional homogeneity which is typically absent in primary cultures. Because of their relative homogeneity, ‘lines’ are capable of being characterized and stably propagated over a period of time. A new *type* of cell line can be established not only through the passaging/selection of a primary culture, but also through experimental modifications of existing lines (e.g. immortalization, stable genetic modifications, drug selection for a resistant subset, etc.). The definition provided here establishes the 'scale' of cell populations that qualify as cell lines - specifically those with a shared propagation history in culture. In this way, the 'cell line' class demarcates populations that represent what researchers actually use in the practice of science - e.g. as inputs to culturing, experimentation, and sharing. The definition is such that cell lines will exhibit important attributes. For example, they will have a relatively homogenous cell type composition as they have experienced similar selective pressures due to their continuous co-propagation. In addition, these populations can also be characterized by a passage number, again owing to their common passaging history.  As defined here, 'cell line' can refer to a population of cells in active culture, applied experimentally, or stored in a quiescent state for future use.

ID

http://purl.obolibrary.org/obo/CLO_0000031

comment

The term 'line' is used when a culture has undergone an intentional experimental process to establish a more uniform and stable population of cells (see 'establishing cell line'). This will require one or more passages, but may involve additional selection processes. Through such passaging and/or selection processes, the resulting 'line' attains some level of genetic stability and compositional homogeneity which is typically absent in primary cultures. Because of their relative homogeneity, ‘lines’ are capable of being characterized and stably propagated over a period of time. A new *type* of cell line can be established not only through the passaging/selection of a primary culture, but also through experimental modifications of existing lines (e.g. immortalization, stable genetic modifications, drug selection for a resistant subset, etc.). The definition provided here establishes the 'scale' of cell populations that qualify as cell lines - specifically those with a shared propagation history in culture. In this way, the 'cell line' class demarcates populations that represent what researchers actually use in the practice of science - e.g. as inputs to culturing, experimentation, and sharing. The definition is such that cell lines will exhibit important attributes. For example, they will have a relatively homogenous cell type composition as they have experienced similar selective pressures due to their continuous co-propagation. In addition, these populations can also be characterized by a passage number, again owing to their common passaging history.  As defined here, 'cell line' can refer to a population of cells in active culture, applied experimentally, or stored in a quiescent state for future use.

editor note

In the spring of 2013, a working group comprised of domain experts and representatives from CLO, OBI, CL, and ReO worked to establish a consensus model and definitions of cultured cells across these efforts. This included a careful characterization of how the term 'cell line' should be defined and applied. Notes about this work and its outcomes can be found on the CLO wiki here: http://code.google.com/p/clo-ontology/wiki/Cell_Lines

editor preferred term

cell line

example of usage

He, Tong-Chuan, et al., Identification of c-MYC as a target of the APC pathway. Science 281.5382 (1998): 1509-1512.: "To evaluate the transcriptional effects of APC, we studied a human colorectal cancer cell line (HT29-APC) containing a zinc-inducible APC gene and a control cell line (HT29–β-Gal) containing an analogous inducible lacZ gene". Note that common usage in the literature is often of the form "a human colorectal cancer cell line", as seen above. But such references to studies in "a line" refer to the fact that discrete populations of cells that are input into culturing or experiments, not an entire lineage of cells. It is these discrete populations that we refer to as 'cell lines'.

imported from

http://purl.obolibrary.org/obo/obi.owl

http://purl.obolibrary.org/obo/clo.owl

label

cell line

prefixIRI

CLO:0000031

prefLabel

cell line

term editor

MB, SS, JZ, MAH, BP, CS, YH

textual definition

A cultured cell population that represents a genetically stable and homogenous population of cultured cells that shares a common propagation history (i.e. has been successively passaged together in culture).

A cultured cell population that represents a genetically stable and homogenous population of cultured cells that shares a common propagation history (i.e. has been successively passaged together in culture).

subClassOf

http://www.bioassayontology.org/bao#BAO_0003114

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Delete Mapping To Ontology Source
http://purl.obolibrary.org/obo/CLO_0000031 PDRO SAME_URI
http://purl.obolibrary.org/obo/CLO_0000031 CLO SAME_URI
http://purl.obolibrary.org/obo/CLO_0000031 OBI SAME_URI
http://purl.obolibrary.org/obo/CLO_0000031 OHPI SAME_URI
http://purl.obolibrary.org/obo/CLO_0000031 HOIP SAME_URI
http://purl.obolibrary.org/obo/CLO_0000031 OMIT SAME_URI
http://purl.obolibrary.org/obo/CLO_0000031 ONE SAME_URI
http://purl.obolibrary.org/obo/CLO_0000031 OBI_BCGO SAME_URI
http://purl.obolibrary.org/obo/CLO_0000031 EGO SAME_URI
http://purl.obolibrary.org/obo/CLO_0000031 OBIB SAME_URI
http://purl.obolibrary.org/obo/CLO_0000031 BERO SAME_URI
http://purl.obolibrary.org/obo/CLO_0000031 OBI_IEE SAME_URI
http://purl.obolibrary.org/obo/CLO_0000031 KTAO SAME_URI
http://purl.obolibrary.org/obo/CLO_0000031 DIDEO SAME_URI
http://purl.obolibrary.org/obo/CLO_0000031 GENEPIO SAME_URI
http://purl.obolibrary.org/obo/CLO_0000031 GENO SAME_URI
http://purl.obolibrary.org/obo/CLO_0000031 NCRO SAME_URI
http://purl.obolibrary.org/obo/CLO_0000031 ERO SAME_URI
http://purl.bioontology.org/ontology/MESH/D002460 MESH LOOM
http://purl.obolibrary.org/obo/MCBCC_0000070#CellLine MCBCC LOOM
http://purl.org/zonmw/covid19/10170 ZONMW-CONTENT LOOM
http://sbmi.uth.tmc.edu/ontology/ochv#C0007600 OCHV LOOM
http://phenomebrowser.net/ontologies/mesh/mesh.owl#D002460 RH-MESH LOOM
http://id.nlm.nih.gov/mesh/D002460 MDM LOOM
http://purl.obolibrary.org/obo/CLO_0000031 PDRO LOOM
http://purl.obolibrary.org/obo/CLO_0000031 CLO LOOM
http://purl.obolibrary.org/obo/CLO_0000031 OBI LOOM
http://purl.obolibrary.org/obo/CLO_0000031 OHPI LOOM
http://purl.obolibrary.org/obo/CLO_0000031 HOIP LOOM
http://purl.obolibrary.org/obo/CLO_0000031 OMIT LOOM
http://purl.obolibrary.org/obo/CLO_0000031 ONE LOOM
http://purl.obolibrary.org/obo/CLO_0000031 OBI_BCGO LOOM
http://purl.obolibrary.org/obo/CLO_0000031 EGO LOOM
http://purl.obolibrary.org/obo/CLO_0000031 OBIB LOOM
http://purl.obolibrary.org/obo/CLO_0000031 OBI_IEE LOOM
http://purl.obolibrary.org/obo/CLO_0000031 KTAO LOOM
http://purl.obolibrary.org/obo/CLO_0000031 DIDEO LOOM
http://purl.obolibrary.org/obo/CLO_0000031 GENEPIO LOOM
http://purl.obolibrary.org/obo/CLO_0000031 GENO LOOM
http://purl.obolibrary.org/obo/CLO_0000031 NCRO LOOM
http://purl.obolibrary.org/obo/CLO_0000031 ERO LOOM
http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C16403 NCIT LOOM
http://uri.neuinfo.org/nif/nifstd/birnlex_11036 NIFSTD LOOM
http://www.w3.org/2001/sw/hcls/ns/transmed/TMO_0036 TMO LOOM
http://purl.obolibrary.org/obo/ID_0000020 BCTEO LOOM
https://w3id.org/reproduceme#CellLine REPRODUCE-ME LOOM
http://mged.sourceforge.net/ontologies/MGEDOntology.owl#CellLine MO LOOM
http://bioontology.org/projects/ontologies/birnlex#birnlex_11036 BIRNLEX LOOM
http://purl.obolibrary.org/obo/OMIT_0003789 OMIT LOOM
http://phenomebrowser.net/ontologies/mesh/mesh.owl#A11.251.210 RH-MESH LOOM
http://jermontology.org/ontology/JERMOntology#Cell_line JERM LOOM
http://ccdb.ucsd.edu/SAO/1.2#sao938874695 SAO LOOM
http://semanticscience.org/resource/SIO_010054 HASCO LOOM
http://semanticscience.org/resource/SIO_010054 HHEARP LOOM
http://semanticscience.org/resource/SIO_010054 SIO LOOM
http://semanticscience.org/resource/SIO_010054 CHEAR LOOM
http://semanticscience.org/resource/SIO_010054 BIOMO LOOM
http://semanticscience.org/resource/SIO_010054 SCO LOOM
http://semanticscience.org/resource/SIO_010054 HHEAR LOOM
http://semanticscience.org/resource/SIO_010054 POEM LOOM
http://purl.bioontology.org/ontology/CSP/4000-0369 CRISP LOOM
http://regenbase.org/ontology#RB_0000170 RB LOOM
http://purl.jp/bio/4/id/200906016774956271 IOBC LOOM
http://www.owl-ontologies.com/NPOntology.owl#CellLine NATPRO LOOM
https://w3id.org/biolink/vocab/CellLine BIOLINK LOOM
http://chem.deri.ie/granatum#Cell_line CANCO LOOM
https://purl.dsmz.de/schema/CellLine D3O LOOM
http://sbmi.uth.tmc.edu/ontology/ochv#2591 OCHV LOOM
http://scai.fraunhofer.de/CSEO#Cell_line CSEO LOOM
https://w3id.org/gold.vocab/Cell-Line GOLDTERMS LOOM
http://www.phoc.org.cn/pmo/class/PMO_00000621 PMAPP-PMO LOOM
http://purl.obolibrary.org/obo/MESH_D002460 BERO LOOM
http://purl.bioontology.org/net/brunel/panda#CellLine PANDA LOOM

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